lentivirus packaging vectors Search Results


psih1-h1-copgfp-sh-nc  (Shanghai GenePharma)
90
Shanghai GenePharma psih1-h1-copgfp-sh-nc
Silencing of GAS5 ameliorates IVDD in vivo by miR-17-3p-mediated inhibition of Ang-2. (a) Observation of the MRI signal intensity and IVDD degree by MRI scanning. (b) Western blot analysis of Ang-2 and ECM metabolism-related proteins in NP tissues extracted from IVDD mice, which was normalized to GAPDH. (c) Statistical results of TUNEL-positive cells in the NP tissues extracted from IVDD mice. (d) The expression of glucosaminoglycan (GAG) in mouse NP tissues detected by Safranin O-Fast Green staining. n = 8. (e) HE histological scores of mouse NP tissues by HE staining. n = 8. ∗ p < 0.05 vs. mice without any injection; # p < 0.05 vs. IVDD mice injected with harboring shRNA against shRNA NC; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring shRNA against GAS5; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring overexpression vector NC; @ p < 0.05 vs. IVDD mice injected with lentivirus harboring oe-GAS5. Data (mean ± standard deviation) were assessed by one-way ANOVA, followed by Tukey's post hoc test. n = 8.
Psih1 H1 Copgfp Sh Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentivirus+packaging+vectors/psih1+h1+copgfp+vector+based+lentivirus+packaging+system/pmc08941580-48-30-21
Average 90 stars, based on 1 article reviews
psih1-h1-copgfp-sh-nc - by Bioz Stars, 2026-09
90/100 stars
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third-generation lentivirus packaging system (vector: packaging plasmid: envelope ratio = 10:3:1  (Promega)
90
Promega third-generation lentivirus packaging system (vector: packaging plasmid: envelope ratio = 10:3:1
Silencing of GAS5 ameliorates IVDD in vivo by miR-17-3p-mediated inhibition of Ang-2. (a) Observation of the MRI signal intensity and IVDD degree by MRI scanning. (b) Western blot analysis of Ang-2 and ECM metabolism-related proteins in NP tissues extracted from IVDD mice, which was normalized to GAPDH. (c) Statistical results of TUNEL-positive cells in the NP tissues extracted from IVDD mice. (d) The expression of glucosaminoglycan (GAG) in mouse NP tissues detected by Safranin O-Fast Green staining. n = 8. (e) HE histological scores of mouse NP tissues by HE staining. n = 8. ∗ p < 0.05 vs. mice without any injection; # p < 0.05 vs. IVDD mice injected with harboring shRNA against shRNA NC; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring shRNA against GAS5; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring overexpression vector NC; @ p < 0.05 vs. IVDD mice injected with lentivirus harboring oe-GAS5. Data (mean ± standard deviation) were assessed by one-way ANOVA, followed by Tukey's post hoc test. n = 8.
Third Generation Lentivirus Packaging System (Vector: Packaging Plasmid: Envelope Ratio = 10:3:1, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentivirus+packaging+vectors/third+generation+lentivirus+packaging+system++vector++packaging+plasmid++envelope+ratio+++10+3+1/ppr0514851-36-10-21
Average 90 stars, based on 1 article reviews
third-generation lentivirus packaging system (vector: packaging plasmid: envelope ratio = 10:3:1 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
lentivirus vectors packaging overexpression plasmid of vmp1 (oe-vmp1)  (VectorBuilder GmbH)
90
VectorBuilder GmbH lentivirus vectors packaging overexpression plasmid of vmp1 (oe-vmp1)
Silencing of GAS5 ameliorates IVDD in vivo by miR-17-3p-mediated inhibition of Ang-2. (a) Observation of the MRI signal intensity and IVDD degree by MRI scanning. (b) Western blot analysis of Ang-2 and ECM metabolism-related proteins in NP tissues extracted from IVDD mice, which was normalized to GAPDH. (c) Statistical results of TUNEL-positive cells in the NP tissues extracted from IVDD mice. (d) The expression of glucosaminoglycan (GAG) in mouse NP tissues detected by Safranin O-Fast Green staining. n = 8. (e) HE histological scores of mouse NP tissues by HE staining. n = 8. ∗ p < 0.05 vs. mice without any injection; # p < 0.05 vs. IVDD mice injected with harboring shRNA against shRNA NC; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring shRNA against GAS5; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring overexpression vector NC; @ p < 0.05 vs. IVDD mice injected with lentivirus harboring oe-GAS5. Data (mean ± standard deviation) were assessed by one-way ANOVA, followed by Tukey's post hoc test. n = 8.
Lentivirus Vectors Packaging Overexpression Plasmid Of Vmp1 (Oe Vmp1), supplied by VectorBuilder GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentivirus+packaging+vectors/lentivirus+vectors+packaging+overexpression+plasmid+of+vmp1++oe+vmp1+/pm37748280-57-7-20
Average 90 stars, based on 1 article reviews
lentivirus vectors packaging overexpression plasmid of vmp1 (oe-vmp1) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Silencing of GAS5 ameliorates IVDD in vivo by miR-17-3p-mediated inhibition of Ang-2. (a) Observation of the MRI signal intensity and IVDD degree by MRI scanning. (b) Western blot analysis of Ang-2 and ECM metabolism-related proteins in NP tissues extracted from IVDD mice, which was normalized to GAPDH. (c) Statistical results of TUNEL-positive cells in the NP tissues extracted from IVDD mice. (d) The expression of glucosaminoglycan (GAG) in mouse NP tissues detected by Safranin O-Fast Green staining. n = 8. (e) HE histological scores of mouse NP tissues by HE staining. n = 8. ∗ p < 0.05 vs. mice without any injection; # p < 0.05 vs. IVDD mice injected with harboring shRNA against shRNA NC; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring shRNA against GAS5; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring overexpression vector NC; @ p < 0.05 vs. IVDD mice injected with lentivirus harboring oe-GAS5. Data (mean ± standard deviation) were assessed by one-way ANOVA, followed by Tukey's post hoc test. n = 8.

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Depleted Long Noncoding RNA GAS5 Relieves Intervertebral Disc Degeneration via microRNA-17-3p/Ang-2

doi: 10.1155/2022/1792412

Figure Lengend Snippet: Silencing of GAS5 ameliorates IVDD in vivo by miR-17-3p-mediated inhibition of Ang-2. (a) Observation of the MRI signal intensity and IVDD degree by MRI scanning. (b) Western blot analysis of Ang-2 and ECM metabolism-related proteins in NP tissues extracted from IVDD mice, which was normalized to GAPDH. (c) Statistical results of TUNEL-positive cells in the NP tissues extracted from IVDD mice. (d) The expression of glucosaminoglycan (GAG) in mouse NP tissues detected by Safranin O-Fast Green staining. n = 8. (e) HE histological scores of mouse NP tissues by HE staining. n = 8. ∗ p < 0.05 vs. mice without any injection; # p < 0.05 vs. IVDD mice injected with harboring shRNA against shRNA NC; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring shRNA against GAS5; $ p < 0.05 vs. IVDD mice injected with lentivirus harboring overexpression vector NC; @ p < 0.05 vs. IVDD mice injected with lentivirus harboring oe-GAS5. Data (mean ± standard deviation) were assessed by one-way ANOVA, followed by Tukey's post hoc test. n = 8.

Article Snippet: Afterwards, plasmids of sh-NC, sh-GAS5, oe-NC, oe-GAS5, miR-17-3p mimic, and miR-17-3p inhibitor were packaged into lentivirus vectors (pSIH1-H1-copGFP and pLV-EGFP-N) by GenePharma Co., Ltd. (Shanghai, China) to generate lentiviruses including pSIH1-H1-copGFP-sh-NC, pSIH1-H1-copGFP-sh-GAS5, pLV-EGFP-NC, pLV-EGFP-GAS5, pSIH1-H1-copGFP-miR-17-3p inhibitor, and pLV-EGFP-miR-17-3p, respectively.

Techniques: In Vivo, Inhibition, Western Blot, TUNEL Assay, Expressing, Staining, Injection, shRNA, Over Expression, Plasmid Preparation, Standard Deviation